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The S-CB7 was highly immunogenic in eliciting protective antibody against the type 24 6RA streptococcus. Lymphocytes from the immunized rabbits responded to heterologous types 5 and 6 streptococcal M proteins as well as the homologous type 24, suggesting that cellular immunity is directed against a common M protein structural determinant. Indications are that only small segments of the M protein are needed to evoke protective immunity. Other data suggested that the available monoclonal antibodies against M proteins may be used to map the protective, and perhaps the tissue cross- reactive determinants of streptococcal M proteins. 7-3 5 ROl AI 16624-04 J. K. Spltznagel (Emory University) : Dr. Spitznagel and co- workers have completed a study of the clinical aspects of arthritis due to GBS in a rat model. Pathology studies show that streptococcal antigen in rat joints tends to be associated with granulocytic infiltration in acutely inflammed soft tissue. Arthropathogenicity of whole GBS cells is essentially intermediate between that of Group A streptococci, which fail to produce disease, and Group D streptococci, which produce disease more quickly and of much shorter duration than disease due to Group B streptococci. Group A streptococci are very re- sistant to lysozyme, Group D are very sensitive, and GBS are intermediate. Heat killed whole GBS type III cells induced rat arthritis with a lag period of 7 days, whereas sonicated bacteria induced similar effects in 24 hours. Evidence suggests that the alternate complement pathway may be involved in the inflamma- tory response to bacterial cells; host lysozyme was also shown to process the streptococcal cells vn vitro to a potentially more toxic form. These studies have contributed Buy Elimite Cream further to an understanding of experimental inflammatory responses to GBS. Hospital Associated Infections 2 ROl AI 19046-02 R. A. Garibaldi (University of Permethrin 5 Percent Elimite Connecticut Health Center) : This project focuses on the need for a better understanding Elimite Cream Scabies of the pathogenesis of postoperative infections. A striking association was shown between infectious complications and age, Elimite Generic surgery duration, and severity of underlying disease. The class of surgical wound correlated with the acquisition of postoperative wound infection: e.g., male, preoperative Elimite For Lice stay and operative site correlated with postoperative respiratory infections, and female, surgical site correlated with post operative urinary tract infection. Accepted guidelines have been widely published for parenteral antibiotic use as prophylaxis against postoperative surgical infections. Despite these guidelines, many surgeons do Elimite 5 Cream not adhere to the recommendations. Unnecessary, prolonged or inappropriate antibiotic usage, according to the study data, has tremendous economic impact on excessive hospi- talization costs. More importantly, patients who received inappropriate prophylaxis were more likely Permethrin Elimite Cream to acquire infection with antibiotic resistant bacteria. Microorganisms such as S^. aureus , enterococci and enterobacter species were the predominant offenders. Study of intraoperative wound cultures, it is believed, is an excellent method to identify high-risk patients. 5 ROl AI 10108-13 A. I. Braude (University of California, San Diego) : This investigator and co-workers have been studying the effect of E^. coli J5 antiserum for therapy and prophylaxis of gram-negative bacteremia in immunocompromised patients. Studies are now underway in patients with bone marrow transplants and in leukemia patients to determine the prophylactic effectiveness of the J5 antiserum. IgM is the protective immunoglobulin against bacteremia, but it was found to have a very short half-life. Monoclonal antibodies to £. coli -0111 LPS and to the J5 LPS were generated. The anti-0111 and the anti-J5 monoclonal antibodies protected vn vivo by preventing death in mice from 0111 LPS and the dermal Elimite Otc Shwartzman reaction in rabbits, and j£ vitro by decreasing the B cell proliferation response to 0111 and J5 LPS. These studies with monoclonal anti- bodies to several Elimite Or Kwell LPS determinants offer a useful tool for unraveling the mechanism of protection by core LPS antiserum in man. 7-4 Cystic Fibrosis 5 ROl AI 15835-04A1 G. B. Pier (Brigham and Women's Hospital) : A vaccine to prevent the severe effects of chronic lung infection in cystic fibrosis Where Can I Buy Elimite patients has long been needed. Dr. Pier has investigated the human Immune Elimite Cream Over The Counter response in volunteers to vaccination with a high M.W. polysaccharide from a Pseudomonas aeruginosa immunotype (IT-1). Levels of specific immunoglobulin to IT-1 in the volunteers remained Permethrin 5 Cream Elimite significantly elevated 21 months post-immunization. Several of the individuals responded to the IT-2 and IT-5 serotypes, but none responded to serotypes IT-3, 4, 6, or 7. Immune serum IT-1 specific Elimite Cream Otc antibody was of the IgG, IgA, and IgM classes. Purified serum IgG and IgA mediated high levels of killing of P_. aeruginosa by peripheral blood leukocytes; complement potentiated the IgG-mediated bactericidal effect Elimite Cost but had no effect on IgA mediated bacterial killing. The data suggest that the P^. Elimite Lice aeruginosa polysaccharide vaccine induces a long lived, serotype specific immune Generic Elimite response. Opsonophagocytosis of the pseudomonas by serum IgA and mononuclear cells suggests that immune IgA and monocytes may be an important protective mechanism for Elimite Price the immunocompromised host. 5 R23 AI 18215-02 Permethrin 5 Elimite K. B. Adler (University of Vermont) : This young investigator has established that P. aeruginosa elaborates a product that stimulates secretion of mucin by explants of both human and rodent trachea in organ cultures. The heat-stable stimulatory effect is not related to the mucoid character of the bacterial isolates nor Permethrin Elimite their serotype. Current data show that stimulation of mucin secretion by the bacterial filtrates may involve binding to ganglioside receptors on the cell surface, prior to stimulation of secretion. Calcium ion
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